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  • Featured Product
  • KD/KO Validated

PELI1 Polyclonal antibody, PBS Only

PELI1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12053-1-PBS

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Synonyms

验证数据展示

  • IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 12053-1-AP (same clone as 12053-1-PBS)

    WB analysis of THP-1 using 12053-1-AP (same clone as 12053-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12053-1-AP (PELI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 12053-1-AP (same clone as 12053-1-PBS)

    IP experiment of SH-SY5Y using 12053-1-AP (same clone as 12053-1-PBS)

    IP result of anti-PELI1 (IP:12053-1-AP, 4ug; Detection:12053-1-AP 1:300) with SH-SY5Y cells lysate 3000ug. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

产品信息

12053-1-PBS targets PELI1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PELI1 fusion protein Ag2683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pellino homolog 1 (Drosophila)
Synonyms
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 46-53 kDa
GenBank Accession NumberBC011419
Gene Symbol PELI1
Gene ID (NCBI) 57162
RRIDAB_2161180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FA3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.