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验证数据展示

  • WB analysis using 30135-1-AP

    WB analysis using 30135-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30135-1-AP (PELP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 30135-1-AP

    IF Staining of MCF-7 using 30135-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PELP1 antibody (30135-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse testis using 30135-1-AP

    IHC staining of mouse testis using 30135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELP1 antibody included
  • IHC staining of mouse testis using 30135-1-AP

    IHC staining of mouse testis using 30135-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PELP1 antibody included

PELP1 Polyclonal antibody

PELP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30135-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inmouse testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PELP1 Ready-To-Use IHC Kit. PELP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30135-1-AP targets PELP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 PELP1 fusion protein Ag32720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline, glutamate and leucine rich protein 1
别名 HMX3, MNAR, P160, PELP1, Transcription factor HMX3
计算分子量 1130 aa, 120 kDa
观测分子量 160 kDa
GenBank蛋白编号BC069058
基因名称 PELP1
Gene ID (NCBI) 27043
RRIDAB_2935519
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

实验方案

Product Specific Protocols
WB protocol for PELP1 antibody 30135-1-APDownload protocol
IHC protocol for PELP1 antibody 30135-1-APDownload protocol
IF protocol for PELP1 antibody 30135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols