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Twinkle/PEO1 Polyclonal antibody

Twinkle/PEO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18793-1-AP

Print datasheet

Synonyms

ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL

验证数据展示

  • WB analysis using 18793-1-AP

    WB analysis using 18793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18793-1-AP (Twinkle antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18793-1-AP

    IHC staining of human pancreas using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

经过测试的应用

Positive WB detected inDaudi cells, MCF-7 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18793-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Twinkle/PEO1 fusion protein Ag6628 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 2
Synonyms ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL
Calculated Molecular Weight 684 aa, 77 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC033762
Gene Symbol PEO1
Gene ID (NCBI) 56652
RRIDAB_2066354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Twinkle, also named as Progressive external ophthalmoplegia 1 protein is a 684 amino acid protein, which involves in mitochondrial DNA (mtDNA) metabolism. The predicted full-length protein is 684 amino acids, with amolecular mass of 77 kD, whereas the splice variant mRNAencodes a 66-kD product of 582 amino acids, lacking residues 579-684 and terminating with four unique amino acids (PMID: 11431692).

实验方案

Product Specific Protocols
WB protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
IHC protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols