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验证数据展示

  • WB analysis using 18793-1-AP

    WB analysis using 18793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18793-1-AP (Twinkle antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18793-1-AP

    IHC staining of human pancreas using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

Twinkle/PEO1 Polyclonal antibody

Twinkle/PEO1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18793-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, MCF-7 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18793-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Twinkle/PEO1 fusion protein Ag6628 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 2
别名 ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL
计算分子量 684 aa, 77 kDa
观测分子量 60 kDa
GenBank蛋白编号BC033762
基因名称 PEO1
Gene ID (NCBI) 56652
RRIDAB_2066354
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Twinkle, also named as Progressive external ophthalmoplegia 1 protein is a 684 amino acid protein, which involves in mitochondrial DNA (mtDNA) metabolism. The predicted full-length protein is 684 amino acids, with amolecular mass of 77 kD, whereas the splice variant mRNAencodes a 66-kD product of 582 amino acids, lacking residues 579-684 and terminating with four unique amino acids (PMID: 11431692).

实验方案

Product Specific Protocols
WB protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
IHC protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols