验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HEK-293 cells, human placenta tissue, NCCIT cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67202-1-Ig targets PEPD in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | PEPD fusion protein Ag28713 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | peptidase D |
| Synonyms | Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase |
| Calculated Molecular Weight | 493 aa, 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC015027 |
| Gene Symbol | PEPD |
| Gene ID (NCBI) | 5184 |
| RRID | AB_2882495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P12955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEPD antibody 67202-1-Ig | Download protocol |
| IHC protocol for PEPD antibody 67202-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
