PEPD Monoclonal antibody

PEPD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67202-1-Ig

产品说明书

CloneNo. 1H2A1

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, human placenta tissue, NCCIT cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67202-1-Ig targets PEPD in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 PEPD fusion protein Ag28713 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidase D
别名 Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase
计算分子量 493 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC015027
基因名称 PEPD
Gene ID (NCBI) 5184
RRIDAB_2882495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP12955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

实验方案

Product Specific Protocols
WB protocol for PEPD antibody 67202-1-IgDownload protocol
IHC protocol for PEPD antibody 67202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Bioeng Biotechnol

Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.

Authors - Jie Chen
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