验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human brain tissue, mouse kidney tissue, mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13463-1-AP targets PER1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PER1 fusion protein Ag4249 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | period homolog 1 (Drosophila) |
| 别名 | RIGUI, period homolog 1 (Drosophila), PER, KIAA0482, hPER1 |
| 计算分子量 | 1290 aa, 136 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC028207 |
| 基因名称 | PER1 |
| Gene ID (NCBI) | 5187 |
| RRID | AB_2161536 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15534 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PER1 antibody 13463-1-AP | Download protocol |
| IHC protocol for PER1 antibody 13463-1-AP | Download protocol |
| IF protocol for PER1 antibody 13463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Dysfunctional circadian clock accelerates cancer metastasis by intestinal microbiota triggering accumulation of myeloid-derived suppressor cells | ||
Theranostics Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model. | ||
J Transl Med Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy | ||
Biochim Biophys Acta Mol Basis Dis Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome | ||
Int J Mol Sci Uncovering the Novel Role of NR1D1 in Regulating BNIP3-Mediated Mitophagy in Ulcerative Colitis |
