验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PER3 Ready-To-Use IHC Kit. PER3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
产品信息
12550-1-AP targets PER3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PER3 fusion protein Ag3400 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | period homolog 3 (Drosophila) |
| 别名 | period homolog 3 (Drosophila), hPER3, GIG13, Cell growth-inhibiting gene 13 protein |
| 计算分子量 | 1201 aa, 132 kDa |
| 观测分子量 | 132 kDa |
| GenBank蛋白编号 | BC026102 |
| 基因名称 | PER3 |
| Gene ID (NCBI) | 8863 |
| RRID | AB_2161814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PER3 antibody 12550-1-AP | Download protocol |
| IHC protocol for PER3 antibody 12550-1-AP | Download protocol |
| IF protocol for PER3 antibody 12550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats. | ||
Int J Biochem Cell Biol TFEB Regulates PER3 Expression via Glucose-dependent Effects on CLOCK/BMAL1. | ||
Biochem Biophys Res Commun Loss of circadian protein TIMELESS accelerates the progression of cellular senescence. | ||
Adv Sci (Weinh) The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis | ||
Anesth Analg Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium | ||
Cancer Lett Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance |