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PERK/EIF2AK3 Monoclonal antibody, PBS Only

PERK/EIF2AK3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G3F2

Cat no : 68482-1-PBS

Print datasheet

Synonyms

DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, WRS

验证数据展示

  • WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68482-1-PBS in a different storage buffer formulation.

  • WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    WB analysis using 68482-1-Ig (same clone as 68482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68482-1-PBS in a different storage buffer formulation.

产品信息

68482-1-PBS targets PERK/EIF2AK3 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PERK/EIF2AK3 fusion protein Ag18254 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Synonyms DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, WRS
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).