验证数据展示
产品信息
68482-1-PBS targets PERK/EIF2AK3 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | PERK/EIF2AK3 fusion protein Ag18254 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2-alpha kinase 3 |
| 别名 | DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, WRS |
| 计算分子量 | 1116 aa, 125 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC126354 |
| 基因名称 | PERK |
| Gene ID (NCBI) | 9451 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NZJ5 |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).