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验证数据展示

  • WB analysis of Jurkat using 26649-1-AP

    WB analysis of Jurkat using 26649-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26649-1-AP

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26649-1-AP

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 26649-1-AP

    IF Staining of HeLa using 26649-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26649-1-AP (PEX13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PEX13 Polyclonal antibody

PEX13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26649-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

NALD, Peroxin 13, PEX13, ZWS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

26649-1-AP targets PEX13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 PEX13 fusion protein Ag24400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 13
别名 NALD, Peroxin 13, PEX13, ZWS
计算分子量 403 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC067090
基因名称 PEX13
Gene ID (NCBI) 5194
RRIDAB_2880588
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92968
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEX13 antibody 26649-1-APDownload protocol
IHC protocol for PEX13 antibody 26649-1-APDownload protocol
IF protocol for PEX13 antibody 26649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Authors - Michael S Dahabieh
View Article
humanWB

Biol Chem

Comparison of human PEX knockout cell lines suggests a dual role of PEX1 in peroxisome biogenesis

Authors - Julia Ott
View Article
humanWB

Nat Commun

Modulation of peroxisomal import by the PEX13 SH3 domain and a proximal FxxxF binding motif

Authors - Stefan Gaussmann
View Article