• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PEX14 Recombinant antibody, PBS Only

PEX14 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7E6

Cat no : 80234-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

验证数据展示

  • WB analysis using 80234-1-RR (same clone as 80234-1-PBS)

    WB analysis using 80234-1-RR (same clone as 80234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80234-1-RR (PEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

    IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

    IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80234-1-RR (same clone as 80234-1-PBS)

    IF Staining of HeLa using 80234-1-RR (same clone as 80234-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (80234-1-RR, Clone: 7E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80234-1-PBS

    Affinity and Kinetic Characterization of 80234-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80234-1-RR against Human PEX14 were performed. The affinity constant is 1.96 nM.

产品信息

80234-1-PBS targets PEX14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen PEX14 fusion protein Ag0932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peroxisomal biogenesis factor 14
Synonyms Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein
Calculated Molecular Weight 41 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC006327
Gene Symbol PEX14
Gene ID (NCBI) 5195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75381
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.