验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14816-1-AP targets PEX16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, arabidopsis |
| 免疫原 | PEX16 fusion protein Ag6566 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 16 |
| 别名 | PEX, Peroxin-16, Peroxin 16 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC000467 |
| 基因名称 | PEX16 |
| Gene ID (NCBI) | 9409 |
| RRID | AB_2162250 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX16 antibody 14816-1-AP | Download protocol |
| IF protocol for PEX16 antibody 14816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.
| ||
Nat Commun Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. | ||
Cell Death Differ Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis. | ||
Proc Natl Acad Sci U S A Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells. | ||
Cell Death Differ Peroxisomes protect lymphoma cells from HDAC inhibitor-mediated apoptosis. |