• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68261-1-Ig

    WB analysis using 68261-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68261-1-Ig (PEX16 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

PEX16 Monoclonal antibody

PEX16 Monoclonal Antibody for WB, ELISA
Cat No. 68261-1-Ig

产品说明书

CloneNo. 2D7G4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, ELISA

别名

Peroxin 16, PEX16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68261-1-Ig targets PEX16 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 PEX16 fusion protein Ag6574 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 16
别名 Peroxin 16, PEX16
计算分子量 39 kDa
观测分子量 38 kDa
GenBank蛋白编号BC000467
基因名称 PEX16
Gene ID (NCBI) 9409
RRIDAB_2935346
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y5Y5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).

实验方案

Product Specific Protocols
WB protocol for PEX16 antibody 68261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols