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PEX3 Polyclonal antibody

PEX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30424-1-AP

Print datasheet

Synonyms

PEX, Peroxin-3, Peroxin 3

验证数据展示

  • IHC staining of mouse kidney using 30424-1-AP

    IHC staining of mouse kidney using 30424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30424-1-AP (PEX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30424-1-AP

    IHC staining of mouse kidney using 30424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30424-1-AP (PEX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30424-1-AP

    WB analysis using 30424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30424-1-AP (PEX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells, PC-12 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30424-1-AP targets PEX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PEX3 fusion protein Ag31516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 3
Synonyms PEX, Peroxin-3, Peroxin 3
Calculated Molecular Weight 42 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC014551
Gene Symbol PEX3
Gene ID (NCBI) 8504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.

实验方案

Product Specific Protocols
WB protocol for PEX3 antibody 30424-1-APDownload protocol
IHC protocol for PEX3 antibody 30424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols