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验证数据展示

  • IHC staining of mouse kidney using 30424-1-AP

    IHC staining of mouse kidney using 30424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30424-1-AP (PEX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30424-1-AP

    IHC staining of mouse kidney using 30424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30424-1-AP (PEX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30424-1-AP

    WB analysis using 30424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30424-1-AP (PEX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PEX3 Polyclonal antibody

PEX3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30424-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PEX, Peroxin-3, Peroxin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells, PC-12 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30424-1-AP targets PEX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PEX3 fusion protein Ag31516 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 3
别名 PEX, Peroxin-3, Peroxin 3
计算分子量 42 kDa
观测分子量42 kDa
GenBank蛋白编号BC014551
基因名称 PEX3
Gene ID (NCBI) 8504
RRIDAB_3086310
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that anchored in the peroxisomal membrane and essential for peroxisome biogenesis and integrity. Additionally, Peroxin 3 acts as a docking factor for Peroxin 19 and is required for the import of peroxisomal proteins. Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import.

实验方案

Product Specific Protocols
WB protocol for PEX3 antibody 30424-1-APDownload protocol
IHC protocol for PEX3 antibody 30424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols