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PEX5L Polyclonal antibody

PEX5L Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13084-1-AP

Print datasheet

Synonyms

Peroxin 5 related protein, PEX2 related protein, PEX5 like protein, PEX5 related protein, PEX5L, PEX5R, Pex5Rp, PXR2, PXR2B

验证数据展示

  • WB analysis of mouse brain using 13084-1-AP

    WB analysis of mouse brain using 13084-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13084-1-AP

    IHC staining of mouse brain using 13084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13084-1-AP

    IHC staining of mouse brain using 13084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 13084-1-AP

    IP experiment of mouse brain using 13084-1-AP

    IP result of anti-PEX5L (IP:13084-1-AP, 4ug; Detection:13084-1-AP 1:300) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13084-1-AP targets PEX5L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PEX5L fusion protein Ag3749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal biogenesis factor 5-like
Synonyms Peroxin 5 related protein, PEX2 related protein, PEX5 like protein, PEX5 related protein, PEX5L, PEX5R, Pex5Rp, PXR2, PXR2B
Calculated Molecular Weight 626 aa, 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC036183
Gene Symbol PEX5L
Gene ID (NCBI) 51555
RRIDAB_2877914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEX5L antibody 13084-1-APDownload protocol
IHC protocol for PEX5L antibody 13084-1-APDownload protocol
IP protocol for PEX5L antibody 13084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Mol Psychiatry

Glucocorticoid-glucocorticoid receptor-HCN1 channels reduce neuronal excitability in dorsal hippocampal CA1 neurons.

Authors - Jiwon Kim
View Article