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验证数据展示

  • WB analysis using 24716-1-AP

    WB analysis using 24716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24716-1-AP

    IF Staining of A431 using 24716-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24716-1-AP (PFAS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PFAS Polyclonal antibody

PFAS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24716-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FGAR-AT, FGARAT, FGAR amidotransferase, FGAMS, FGAM synthase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24716-1-AP targets PFAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PFAS fusion protein Ag20162 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoribosylformylglycinamidine synthase
别名 FGAR-AT, FGARAT, FGAR amidotransferase, FGAMS, FGAM synthase
计算分子量 1338 aa, 145 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC167158
基因名称 PFAS
Gene ID (NCBI) 5198
RRIDAB_3669446
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15067
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.

实验方案

Product Specific Protocols
WB protocol for PFAS antibody 24716-1-APDownload protocol
IHC protocol for PFAS antibody 24716-1-APDownload protocol
IF protocol for PFAS antibody 24716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols