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验证数据展示

  • IF Staining of HepG2 using 83074-3-RR

    IF Staining of HepG2 using 83074-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PFAS antibody (83074-3-RR, Clone: 230422D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83074-3-RR

    Affinity and Kinetic Characterization of 83074-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83074-3-RR against Human PFAS were performed. The affinity constant is 0.58 nM.

PFAS Recombinant antibody

PFAS Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83074-3-RR

产品说明书

CloneNo. 230422D12

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, ELISA

别名

FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83074-3-RR targets PFAS in IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PFAS fusion protein Ag20162 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoribosylformylglycinamidine synthase
别名 FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL
计算分子量 1338 aa, 145 kDa
GenBank蛋白编号BC167158
基因名称 PFAS
Gene ID (NCBI) 5198
RRIDAB_3670794
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15067
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PFAS antibody 83074-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols