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PFDN4 Polyclonal antibody

PFDN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16045-1-AP

Print datasheet

Synonyms

C1, PFD4, PFDN4, prefoldin 4, prefoldin subunit 4, Protein C 1

验证数据展示

  • WB analysis using 16045-1-AP

    WB analysis using 16045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16045-1-AP (PFDN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 16045-1-AP

    IHC staining of human breast cancer using 16045-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFDN4 antibody included
  • IHC staining of human breast cancer using 16045-1-AP

    IHC staining of human breast cancer using 16045-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16045-1-AP (PFDN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFDN4 antibody included

经过测试的应用

Positive WB detected inMCF-7 cells, Raji cells, THP-1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PFDN4 Ready-To-Use IHC Kit. PFDN4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16045-1-AP targets PFDN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PFDN4 fusion protein Ag8938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prefoldin subunit 4
Synonyms C1, PFD4, PFDN4, prefoldin 4, prefoldin subunit 4, Protein C 1
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC010953
Gene Symbol PFDN4
Gene ID (NCBI) 5203
RRIDAB_2162573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human PFDN4, prefoldin 4, is a subunit of the heterohexameric chaperone protein and belongs to the prefoldin family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. Previous reports have shown that PFDN4 is upregulated in breast cancer cell lines and tumor tissues (PMID: 11381030; PMID: 9630229). PFDN4 expression may be a prognostic factor in colorectal cancer (PMID: 20552408).

实验方案

Product Specific Protocols
WB protocol for PFDN4 antibody 16045-1-APDownload protocol
IHC protocol for PFDN4 antibody 16045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

miR-26a regulates extracellular vesicle secretion from prostate cancer cells via targeting SHC4, PFDN4, and CHORDC1.

Authors - Fumihiko Urabe
View Article
mouseWB,IF

Biomark Res

Single-cell and bulk RNA-seq unveils the immune infiltration landscape associated with cuproptosis in cerebral cavernous malformations

Authors - Chengwei Chen
View Article