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PFDN5 Polyclonal antibody

PFDN5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15078-1-AP

Print datasheet

Synonyms

PFD5, MM1, c-Myc-binding protein Mm-1

验证数据展示

  • WB analysis of HepG2 using 15078-1-AP

    WB analysis of HepG2 using 15078-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15078-1-AP

    IF Staining of HepG2 using 15078-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFDN5 antibody (15078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, mouse pancreas tissue, PC-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15078-1-AP targets PFDN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PFDN5 fusion protein Ag7150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prefoldin subunit 5
Synonyms PFD5, MM1, c-Myc-binding protein Mm-1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC003373
Gene Symbol PFDN5
Gene ID (NCBI) 5204
RRIDAB_2162703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PFDN5 antibody 15078-1-APDownload protocol
IF protocol for PFDN5 antibody 15078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Brain Sci

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest

Authors - Qiang Zhang
View Article