验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse pancreas tissue, PC-3 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:300-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
15078-1-AP targets PFDN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | rat |
免疫原 | PFDN5 fusion protein Ag7150 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | prefoldin subunit 5 |
别名 | PFD5, MM1, c-Myc-binding protein Mm-1 |
计算分子量 | 17 kDa |
观测分子量 | 17 kDa |
GenBank蛋白编号 | BC003373 |
基因名称 | PFDN5 |
Gene ID (NCBI) | 5204 |
RRID | AB_2162703 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99471 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PFDN5 antibody 15078-1-AP | Download protocol |
IF protocol for PFDN5 antibody 15078-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |