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验证数据展示

  • WB analysis using 21718-1-AP

    WB analysis using 21718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21718-1-AP (PFKFB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21718-1-AP targets PFKFB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PFKFB1 fusion protein Ag16362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
别名 6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX
计算分子量 471 aa, 55 kDa
观测分子量 47 kDa
GenBank蛋白编号BC096079
基因名称 PFKFB1
Gene ID (NCBI) 5207
RRIDAB_2878911
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16118
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols