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PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21718-1-AP

Print datasheet

Synonyms

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX

验证数据展示

  • WB analysis using 21718-1-AP

    WB analysis using 21718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21718-1-AP (PFKFB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21718-1-AP is PFKFB1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PFKFB1 fusion protein Ag16362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Synonyms 6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX
Calculated Molecular Weight 471 aa, 55 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC096079
Gene Symbol PFKFB1
Gene ID (NCBI) 5207
RRIDAB_2878911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols