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PFKFB4 Polyclonal antibody

PFKFB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29902-1-AP

Print datasheet

Synonyms

6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4, PFKFB4

验证数据展示

  • WB analysis using 29902-1-AP

    WB analysis using 29902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29902-1-AP

    IHC staining of human kidney using 29902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 29902-1-AP

    IHC staining of human kidney using 29902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 29902-1-AP

    IF Staining of HUVEC using 29902-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using PFKFB4 antibody (29902-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, MDA-MB-231 cells, PC-3 cells, Raji cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29902-1-AP targets PFKFB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen PFKFB4 fusion protein Ag30571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Synonyms 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4, PFKFB4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberNM_001317134
Gene Symbol PFKFB4
Gene ID (NCBI) 5210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16877
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).

实验方案

Product Specific Protocols
WB protocol for PFKFB4 antibody 29902-1-APDownload protocol
IHC protocol for PFKFB4 antibody 29902-1-APDownload protocol
IF protocol for PFKFB4 antibody 29902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols