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  • Featured Product
  • KD/KO Validated

PFKM Polyclonal antibody

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30326-1-AP

Print datasheet

Synonyms

GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase

验证数据展示

  • WB analysis using 30326-1-AP

    WB analysis using 30326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 30326-1-AP

    WB analysis of HEK-293 using 30326-1-AP

    WB result of PFKM antibody (30326-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.

  • IHC staining of mouse skeletal muscle using 30326-1-AP

    IHC staining of mouse skeletal muscle using 30326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKM antibody included
  • IHC staining of mouse skeletal muscle using 30326-1-AP

    IHC staining of mouse skeletal muscle using 30326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKM antibody included
  • IF Staining of HepG2 using 30326-1-AP

    IF Staining of HepG2 using 30326-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed 48-well HepG2 cells using PFKM antibody (30326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, Raji cells, MCF-7 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in48-well HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PFKM Ready-To-Use IHC Kit. PFKM primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30326-1-AP targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PFKM fusion protein Ag30840 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphofructokinase, muscle
Synonyms GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC021203
Gene Symbol PFKM
Gene ID (NCBI) 5213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.

实验方案

Product Specific Protocols
WB protocol for PFKM antibody 30326-1-APDownload protocol
IHC protocol for PFKM antibody 30326-1-APDownload protocol
IF protocol for PFKM antibody 30326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols