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PFKP Polyclonal antibody

PFKP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13389-1-AP

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Synonyms

PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected inJurkat cells, mouse heart tissue
Positive IHC detected inmouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, BxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PFKP Ready-To-Use IHC Kit. PFKP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PFKP fusion protein Ag4047 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphofructokinase, platelet
Synonyms PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type
Calculated Molecular Weight 784 aa, 86 kDa
Observed Molecular Weight 80-86 kDa
GenBank Accession NumberBC029138
Gene Symbol PFKP
Gene ID (NCBI) 5214
RRIDAB_2252278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

实验方案

Product Specific Protocols
WB protocol for PFKP antibody 13389-1-APDownload protocol
IHC protocol for PFKP antibody 13389-1-APDownload protocol
IF protocol for PFKP antibody 13389-1-APDownload protocol
IP protocol for PFKP antibody 13389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Cell Metab

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Authors - Xiaolei Lin
mouseWB,IF

Nat Metab

Time of exercise differentially impacts bone growth in mice

Authors - Shaoling Yu
humanWB

Mol Cell

R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.

Authors - Ying Qing
  • KD Validated
humanWB

Stem Cell Reports

The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.

Authors - Zihao Sun
mouseWB

Acta Pharmacol Sin

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.

Authors - Lei Xu