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PFKP Polyclonal antibody
PFKP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells |
Positive IP detected in | Jurkat cells, mouse heart tissue |
Positive IHC detected in | mouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, BxPC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PFKP fusion protein Ag4047 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phosphofructokinase, platelet |
Synonyms | PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type |
Calculated Molecular Weight | 784 aa, 86 kDa |
Observed Molecular Weight | 80-86 kDa |
GenBank Accession Number | BC029138 |
Gene Symbol | PFKP |
Gene ID (NCBI) | 5214 |
RRID | AB_2252278 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q01813 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PFKP antibody 13389-1-AP | Download protocol |
IHC protocol for PFKP antibody 13389-1-AP | Download protocol |
IF protocol for PFKP antibody 13389-1-AP | Download protocol |
IP protocol for PFKP antibody 13389-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Mol Cell R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.
| ||
Stem Cell Reports The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway. | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. |