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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

    WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

PGAM5 Monoclonal antibody, PBS Only

PGAM5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68116-1-PBS

产品说明书

CloneNo. 1C8B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit

应用

WB, IHC, ELISA

别名

BXLBv68, PGAM5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68116-1-PBS targets PGAM5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit
免疫原 PGAM5 fusion protein Ag28195 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglycerate mutase family member 5
别名 BXLBv68, PGAM5
计算分子量 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC008196
基因名称 PGAM5
Gene ID (NCBI) 192111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96HS1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.