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PGAM5 Monoclonal antibody, PBS Only

PGAM5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8B9

Cat no : 68116-1-PBS

Print datasheet

Synonyms

BXLBv68, PGAM5

验证数据展示

  • WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

    WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

产品信息

68116-1-PBS targets PGAM5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen PGAM5 fusion protein Ag28195 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoglycerate mutase family member 5
Synonyms BXLBv68, PGAM5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC008196
Gene Symbol PGAM5
Gene ID (NCBI) 192111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HS1
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.