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PGBD3 Polyclonal antibody

PGBD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17639-1-AP

Print datasheet

Synonyms

PGBD3

验证数据展示

  • WB analysis of HeLa using 17639-1-AP

    WB analysis of HeLa using 17639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17639-1-AP (PGBD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17639-1-AP

    IHC staining of human kidney using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 17639-1-AP

    IHC staining of human kidney using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17639-1-AP targets PGBD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PGBD3 fusion protein Ag11766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name piggyBac transposable element derived 3
Synonyms PGBD3
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC063690
Gene Symbol PGBD3
Gene ID (NCBI) 267004
RRIDAB_2262195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PGBD3 antibody 17639-1-APDownload protocol
IHC protocol for PGBD3 antibody 17639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols