PGC Polyclonal antibody

PGC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28532-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

Pepsinogen II, PGⅡ, EC:3.4.23.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PGC fusion protein Ag29347 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 progastricsin (pepsinogen C)
别名 Pepsinogen II, PGⅡ, EC:3.4.23.3
计算分子量 388 aa, 42 kDa
GenBank蛋白编号BC073740
基因名称 PGC
Gene ID (NCBI) 5225
RRIDAB_2918173
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20142
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PGC antibody 28532-1-APDownload protocol
IHC protocol for PGC antibody 28532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Biosci

Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy

Authors - Mei-Fei Wu
humanWB

Transgenic Res

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells

Authors - Hua-Chuan Zheng
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