PGC Polyclonal antibody

PGC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, MOUSE, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28532-1-AP

Print datasheet

Synonyms

Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)



经过测试的应用

Positive WB detected inmouse stomach tissue, rat stomach tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with Human, MOUSE, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, MOUSE, rat
Cited Reactivityhuman, mouse
Immunogen PGC fusion protein Ag29347 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progastricsin (pepsinogen C)
Synonyms Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)
Calculated Molecular Weight 388 aa, 42 kDa
GenBank Accession NumberBC073740
Gene Symbol PGC
Gene ID (NCBI) 5225
RRIDAB_2918173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20142
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PGC antibody 28532-1-APDownload protocol
IHC protocol for PGC antibody 28532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Biosci

Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy

Authors - Mei-Fei Wu
humanWB

Transgenic Res

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells

Authors - Hua-Chuan Zheng