验证数据展示
经过测试的应用
Positive WB detected in | mouse stomach tissue, rat stomach tissue |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | PGC fusion protein Ag29347 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | progastricsin (pepsinogen C) |
别名 | Pepsinogen II, PGⅡ, EC:3.4.23.3 |
计算分子量 | 388 aa, 42 kDa |
GenBank蛋白编号 | BC073740 |
基因名称 | PGC |
Gene ID (NCBI) | 5225 |
RRID | AB_2918173 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P20142 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for PGC antibody 28532-1-AP | Download protocol |
IHC protocol for PGC antibody 28532-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy | ||
Transgenic Res The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells |