验证数据展示
经过测试的应用
| Positive WB detected in | mouse stomach tissue, rat stomach tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | PGC fusion protein Ag29347 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | progastricsin (pepsinogen C) |
| Synonyms | Pepsinogen II, PGⅡ, EC:3.4.23.3 |
| Calculated Molecular Weight | 388 aa, 42 kDa |
| GenBank Accession Number | BC073740 |
| Gene Symbol | PGC |
| Gene ID (NCBI) | 5225 |
| RRID | AB_2918173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20142 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PGC antibody 28532-1-AP | Download protocol |
| IHC protocol for PGC antibody 28532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy | ||
Transgenic Res The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells |
