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PGK1 Monoclonal antibody, PBS Only

PGK1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2F3

Cat no : 68035-1-PBS

Print datasheet

Synonyms

PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein

验证数据展示

  • WB analysis using 68035-1-Ig (same clone as 68035-1-PBS)

    WB analysis using 68035-1-Ig (same clone as 68035-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68035-1-Ig (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGK1 antibody included
  • IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy PGK1 Ready-To-Use IHC Kit. PGK1 primary antibody included.

产品信息

68035-1-PBS targets PGK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PGK1 fusion protein Ag12099 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoglycerate kinase 1
Synonyms PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC103752
Gene Symbol PGK1
Gene ID (NCBI) 5230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00558
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).