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验证数据展示

  • WB analysis using 29686-1-AP

    WB analysis using 29686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29686-1-AP (PGM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 29686-1-AP

    IHC staining of human prostate hyperplasia using 29686-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 29686-1-AP (PGM3 antibody) at dilution of 1:200 (under 10x lens).

PGM3 Polyclonal antibody

PGM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29686-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29686-1-AP targets PGM3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 PGM3 fusion protein Ag30504 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglucomutase 3
别名 AGM1, DKFZp434B187, PAGM, PGM 3, PGM3, phosphoglucomutase 3
计算分子量 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC001258
基因名称 PGM3
Gene ID (NCBI) 5238
RRIDAB_2918339
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PGM3 antibody 29686-1-APDownload protocol
IHC protocol for PGM3 antibody 29686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols