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  • Featured Product
  • KD/KO Validated

PGRMC2 Monoclonal antibody, PBS Only

PGRMC2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F10D7

Cat No : 60249-1-PBS

Print datasheet

Synonyms

DG6, PGRMC2, PMBP

验证数据展示

  • WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

    WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60249-1-Ig (PGRMC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.

产品信息

60249-1-PBS targets PGRMC2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen PGRMC2 fusion protein Ag20077 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name progesterone receptor membrane component 2
Synonyms DG6, PGRMC2, PMBP
Calculated Molecular Weight 223 aa, 24 kDa
Observed Molecular Weight 24+28 kDa
GenBank Accession NumberBC016692
Gene Symbol PGRMC2
Gene ID (NCBI) 10424
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).