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PGT Polyclonal antibody

PGT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30434-1-AP

Print datasheet

Synonyms

SLCO2A1, PHOAR2, OATP2A1

验证数据展示

  • WB analysis using 30434-1-AP

    WB analysis using 30434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30434-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30434-1-AP

    IHC staining of human stomach cancer using 30434-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30434-1-AP (PGT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNIH/3T3 cells, U-251 cells, mouse heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30434-1-AP targets PGT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PGT fusion protein Ag32275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier organic anion transporter family, member 2A1
Synonyms SLCO2A1, PHOAR2, OATP2A1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC041140
Gene Symbol PGT
Gene ID (NCBI) 6578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92959
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).

实验方案

Product Specific Protocols
WB protocol for PGT antibody 30434-1-APDownload protocol
IHC protocol for PGT antibody 30434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols