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PGT Recombinant antibody, PBS Only

PGT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230139F5

Cat no : 83302-1-PBS

Print datasheet

Synonyms

SLCO2A1, PHOAR2, OATP2A1, 230139F5

验证数据展示

  • WB analysis of NIH/3T3 using 83302-1-RR (same clone as 83302-1-PBS)

    WB analysis of NIH/3T3 using 83302-1-RR (same clone as 83302-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 83302-1-RR (same clone as 83302-1-PBS)

    WB analysis of mouse heart using 83302-1-RR (same clone as 83302-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83302-1-RR (same clone as 83302-1-PBS)

    FC experiment of HeLa using 83302-1-RR (same clone as 83302-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGT Recombinant antibody (83302-1-RR, Clone:230139F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83302-1-RR

    Affinity and Kinetic Characterization of 83302-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83302-1-RR against Human PGT were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 83302-1-PBS is PGT Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PGT fusion protein Ag5594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier organic anion transporter family, member 2A1
Synonyms SLCO2A1, PHOAR2, OATP2A1, 230139F5
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC041140
Gene Symbol PGT
Gene ID (NCBI) 6578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92959
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).