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验证数据展示

  • WB analysis of NIH/3T3 using 83302-1-RR (same clone as 83302-1-PBS)

    WB analysis of NIH/3T3 using 83302-1-RR (same clone as 83302-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 83302-1-RR (same clone as 83302-1-PBS)

    WB analysis of mouse heart using 83302-1-RR (same clone as 83302-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83302-1-RR (same clone as 83302-1-PBS)

    FC experiment of HeLa using 83302-1-RR (same clone as 83302-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGT Recombinant antibody (83302-1-RR, Clone:230139F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83302-1-RR

    Affinity and Kinetic Characterization of 83302-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83302-1-RR against Human PGT were performed. The affinity constant is below 1 pM.

PGT Recombinant antibody, PBS Only

PGT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83302-1-PBS

产品说明书

CloneNo. 230139F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

SLCO2A1, PHOAR2, OATP2A1, 230139F5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83302-1-PBS targets PGT in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PGT fusion protein Ag5594 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier organic anion transporter family, member 2A1
别名 SLCO2A1, PHOAR2, OATP2A1, 230139F5
计算分子量 70 kDa
观测分子量 65 kDa
GenBank蛋白编号BC041140
基因名称 PGT
Gene ID (NCBI) 6578
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92959
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).