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验证数据展示

  • WB analysis using 23446-1-AP

    WB analysis using 23446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23446-1-AP (PHACTR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 23446-1-AP

    IF Staining of mouse brain using 23446-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PHACTR1 antibody (23446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PHACTR1 Polyclonal antibody

PHACTR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 23446-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

RPEL1, RPEL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23446-1-AP targets PHACTR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PHACTR1 fusion protein Ag20125 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatase and actin regulator 1
别名 RPEL1, RPEL
计算分子量 580 aa, 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC101526
基因名称 PHACTR1
Gene ID (NCBI) 221692
RRIDAB_2879281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9C0D0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHACTR1 antibody 23446-1-APDownload protocol
IHC protocol for PHACTR1 antibody 23446-1-APDownload protocol
IF protocol for PHACTR1 antibody 23446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Pediatr

Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS

Authors - A Kaitlyn Victor
View Article