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PHAX Polyclonal antibody, PBS Only

PHAX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16481-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    WB analysis of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    IF Staining of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16481-1-AP (PHAX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

产品信息

16481-1-PBS targets PHAX in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PHAX fusion protein Ag9602 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylated adaptor for RNA export
Synonyms
Calculated Molecular Weight 394 aa, 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC021161
Gene Symbol PHAX
Gene ID (NCBI) 51808
RRIDAB_2299663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H814
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Small nuclear and small nucleolar RNAs (snRNAs and snoRNAs) are essential components of snRNPs and snoRNPs, and have a critical role in the maturation of, respectively, mRNAs and rRNAs within the nucleus of eukaryotic cells. Complex and specific pathways exist for the assembly of snRNPs and snoRNPs, involving, nucleocytoplasmic transport of snRNAs and intranuclear transport between compartments of snoRNAs. In metazoa, a subset of spliceosomal snRNAs are exported from the nucleus after transcription. This export occurs in a large complex containing a snRNA, the nuclear cap binding complex (CBC), RanGTP, the leucine-rich nuclear export signal receptor CRM1/Xpo1, and the recently identified phosphoprotein PHAX (phosphorylated adaptor for RNA export). PHAX contains a conserved single-stranded nucleic acid binding domain (RNA_GG_bind domain) with no sequence homology with any other known RNA-binding module [PMID:15574332,11333016, 15574332]