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PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12867-1-AP

Print datasheet

Synonyms

Polyhomeotic-like protein 2, Polyhomeotic like protein 2, PH2, HPH2, EDR2

验证数据展示

  • IHC staining of human pancreas cancer using 12867-1-AP

    IHC staining of human pancreas cancer using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHC2 antibody included
  • IHC staining of human pancreas cancer using 12867-1-AP

    IHC staining of human pancreas cancer using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHC2 antibody included
  • WB analysis of A375 using 12867-1-AP

    WB analysis of A375 using 12867-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 12867-1-AP

    IF Staining of A375 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HL-60 using 12867-1-AP

    IP experiment of HL-60 using 12867-1-AP

    IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:1500) with HL-60 cells lysate 2240 ug.

经过测试的应用

Positive WB detected inA375 cells, A549 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy PHC2 Ready-To-Use IHC Kit. PHC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

12867-1-AP targets PHC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PHC2 fusion protein Ag3507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polyhomeotic homolog 2 (Drosophila)
Synonyms Polyhomeotic-like protein 2, Polyhomeotic like protein 2, PH2, HPH2, EDR2
Calculated Molecular Weight 858 aa, 91 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC028396
Gene Symbol PHC2
Gene ID (NCBI) 1912
RRIDAB_2877890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHC2 antibody 12867-1-APDownload protocol
IHC protocol for PHC2 antibody 12867-1-APDownload protocol
IF protocol for PHC2 antibody 12867-1-APDownload protocol
IP protocol for PHC2 antibody 12867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Mol Cell

Modularity of PRC1 composition and chromatin interaction define condensate properties

Authors - Stefan Niekamp
View Article