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验证数据展示

  • WB analysis using 28472-1-AP

    WB analysis using 28472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 28472-1-AP

    WB analysis of HepG2 using 28472-1-AP

    WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells.

  • IF Staining of HeLa using 28472-1-AP

    IF Staining of HeLa using 28472-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PHF17 Polyclonal antibody

PHF17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28472-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

FLJ22479, JADE1, KIAA1807, PHD finger protein 17, PHF17, Protein Jade 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, A549 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHF17 Ready-To-Use IHC Kit. PHF17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28472-1-AP targets PHF17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PHF17 fusion protein Ag29675 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 17
别名 FLJ22479, JADE1, KIAA1807, PHD finger protein 17, PHF17, Protein Jade 1
计算分子量 96 kDa
观测分子量65, 100-110 kDa
GenBank蛋白编号BC032376
基因名称 PHF17
Gene ID (NCBI) 79960
RRIDAB_2918168
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IE81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 and 100-110 kDa.

实验方案

Product Specific Protocols
WB protocol for PHF17 antibody 28472-1-APDownload protocol
IF protocol for PHF17 antibody 28472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols