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PHF19 Polyclonal antibody
PHF19 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue |
| Positive IF/ICC detected in | Mouse ES cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
11895-1-AP targets PHF19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | PHF19 fusion protein Ag2511 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PHD finger protein 19 |
| Synonyms | Polycomb-like protein 3, Polycomb like protein 3, PHD finger protein 19, PCL3, MTF2L1 |
| Calculated Molecular Weight | 580 aa, 63 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC022374 |
| Gene Symbol | PHF19 |
| Gene ID (NCBI) | 26147 |
| RRID | AB_10804890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T6S3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF19 antibody 11895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.
| ||
PLoS Genet Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. | ||
PLoS Genet Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.
| ||
Front Immunol Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. | ||
J Cancer ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2. | ||
Scand J Gastroenterol Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.
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