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PHF20 Polyclonal antibody, PBS Only

PHF20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22010-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

产品信息

22010-1-PBS targets PHF20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PHF20 fusion protein Ag15686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PHD finger protein 20
Synonyms
Calculated Molecular Weight 1012 aa, 115 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC150178
Gene Symbol PHF20
Gene ID (NCBI) 51230
RRIDAB_2878970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVI0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).