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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

PHF20 Polyclonal antibody, PBS Only

PHF20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22010-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

22010-1-PBS targets PHF20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PHF20 fusion protein Ag15686 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 20
别名
计算分子量 1012 aa, 115 kDa
观测分子量 130 kDa
GenBank蛋白编号BC150178
基因名称 PHF20
Gene ID (NCBI) 51230
RRIDAB_2878970
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVI0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).