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PHF24 Polyclonal antibody

PHF24 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27959-1-AP

Print datasheet

Synonyms

DKFZp434I2112, GINIP, KIAA1045, PHF24, Protein KIAA1045, RP11 392A14.4

验证数据展示

  • IHC staining of mouse brain using 27959-1-AP

    IHC staining of mouse brain using 27959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27959-1-AP

    IHC staining of mouse brain using 27959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27959-1-AP (PHF24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27959-1-AP

    IF Staining of mouse brain using 27959-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 27959-1-AP

    IF Staining of mouse brain using 27959-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PHF24 antibody (27959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27959-1-AP targets PHF24 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PHF24 fusion protein Ag27492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1045
Synonyms DKFZp434I2112, GINIP, KIAA1045, PHF24, Protein KIAA1045, RP11 392A14.4
Calculated Molecular Weight 45 aa
GenBank Accession NumberNM_001304333
Gene Symbol PHF24
Gene ID (NCBI) 23349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PHF24 antibody 27959-1-APDownload protocol
IF protocol for PHF24 antibody 27959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols