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PHF5A Polyclonal antibody

PHF5A Polyclonal Antibody for WB, IP, ELISA
Cat No. 15554-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (4)

bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHF5A Ready-To-Use IHC Kit. PHF5A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15554-1-AP targets PHF5A in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PHF5A fusion protein Ag7918 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 5A
别名 bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b
计算分子量 12 kDa
观测分子量 12-14 kDa
GenBank蛋白编号BC007321
基因名称 PHF5A
Gene ID (NCBI) 84844
RRIDAB_2165365
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7RTV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for PHF5A antibody 15554-1-APDownload protocol
IP protocol for PHF5A antibody 15554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.

Authors - Alexandros Strikoudis
  • KO Validated
humanWB,IP,ChIP,IF

Gastroenterology

RNA Polymerase II-Associated Factor 1 Regulates Stem Cell Features of Pancreatic Cancer Cells, Independently of the PAF1 Complex, via Interactions With PHF5A and DDX3.

Authors - Saswati Karmakar
humanWB

Gastroenterology

Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.

Authors - Yingying Cao
human,mouseWB,CoIP,ChIP

Nat Commun

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics

Authors - Mai Abdel Mouti

Genes Dev

The cryo-EM structure of the SF3b spliceosome complex bound to a splicing modulator reveals a pre-mRNA substrate competitive mechanism of action.

Authors - Lorenzo I Finci
humanWB

Nat Commun

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.

Authors - Teng Teng
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