验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15554-1-AP targets PHF5A in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | PHF5A fusion protein Ag7918 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | PHD finger protein 5A |
别名 | bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b |
计算分子量 | 12 kDa |
观测分子量 | 12-14 kDa |
GenBank蛋白编号 | BC007321 |
基因名称 | PHF5A |
Gene ID (NCBI) | 84844 |
RRID | AB_2165365 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q7RTV0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.
实验方案
Product Specific Protocols | |
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WB protocol for PHF5A antibody 15554-1-AP | Download protocol |
IP protocol for PHF5A antibody 15554-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
| ||
Gastroenterology RNA Polymerase II-Associated Factor 1 Regulates Stem Cell Features of Pancreatic Cancer Cells, Independently of the PAF1 Complex, via Interactions With PHF5A and DDX3. | ||
Gastroenterology Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p. | ||
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Genes Dev The cryo-EM structure of the SF3b spliceosome complex bound to a splicing modulator reveals a pre-mRNA substrate competitive mechanism of action. | ||
Nat Commun Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex. |