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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included
  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included

PHF8 Polyclonal antibody, PBS Only

PHF8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29516-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PHF8 Ready-To-Use IHC Kit. PHF8 primary antibody included.

产品信息

29516-1-PBS targets PHF8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 PHF8 fusion protein Ag30701 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 8
别名
计算分子量 118 kDa
观测分子量125-135 kDa
GenBank蛋白编号NM_001184896
基因名称 PHF8
Gene ID (NCBI) 23133
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPP1
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).