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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included
  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included

PHF8 Polyclonal antibody, PBS Only

PHF8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29516-1-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PHF8 Ready-To-Use IHC Kit. PHF8 primary antibody included.

产品信息

29516-1-PBS targets PHF8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PHF8 fusion protein Ag30701 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PHD finger protein 8
Synonyms
Calculated Molecular Weight 118 kDa
Observed Molecular Weight125-135 kDa
GenBank Accession NumberNM_001184896
Gene Symbol PHF8
Gene ID (NCBI) 23133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPP1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).