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PHGDH Monoclonal antibody, PBS Only

PHGDH Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E8B8

Cat No : 67591-1-PBS

Print datasheet

Synonyms

3 PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, PGDH3, PHGDH, phosphoglycerate dehydrogenase, SERA

验证数据展示

  • IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (67591-1-Ig, Clone: 1E8B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67591-1-Ig (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67591-1-Ig (PHGDH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHGDH antibody included
Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.

产品信息

67591-1-PBS targets PHGDH in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PHGDH fusion protein Ag6877 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoglycerate dehydrogenase
Synonyms 3 PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, PGDH3, PHGDH, phosphoglycerate dehydrogenase, SERA
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC000303
Gene Symbol PHGDH
Gene ID (NCBI) 26227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43175
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).