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验证数据展示

  • WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67591-1-Ig (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IP experiment of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IP result of anti-PHGDH (IP:67591-1-Ig, 4ug; Detection:67591-1-Ig 1:3000) with HeLa cells lysate 980 ug. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67591-1-Ig (PHGDH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHGDH antibody included
  • IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (67591-1-Ig, Clone: 1E8B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

PHGDH Monoclonal antibody, PBS Only

PHGDH Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67591-1-PBS

产品说明书

CloneNo. 1E8B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.

产品信息

67591-1-PBS targets PHGDH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHGDH fusion protein Ag6877 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglycerate dehydrogenase
别名 EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC000303
基因名称 PHGDH
Gene ID (NCBI) 26227
RRIDAB_2882799
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43175
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).