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Species Reactivity
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验证数据展示

  • WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)

    WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)

    WB result of PHGDH antibody (81986-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • WB analysis using 81986-1-RR (same clone as 81986-1-PBS)

    WB analysis using 81986-1-RR (same clone as 81986-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81986-1-RR (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)

    IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)

    IP result of anti-PHGDH (IP:81986-1-RR, 4ug; Detection:81986-1-RR 1:10000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

    IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHGDH antibody included
  • Affinity and Kinetic Characterization of 81986-1-RR

    Affinity and Kinetic Characterization of 81986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81986-1-RR against Human PHGDH were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

PHGDH Recombinant antibody, PBS Only

PHGDH Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81986-1-PBS

Print datasheet

CloneNo. 5L16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 5L16, 3-PGDH, 3PGDH

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.

产品信息

81986-1-PBS targets PHGDH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PHGDH fusion protein Ag6445 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoglycerate dehydrogenase
Synonyms EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 5L16, 3-PGDH, 3PGDH
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC000303
Gene Symbol PHGDH
Gene ID (NCBI) 26227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43175
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).