• 概述
  • 经过测试的应用
  • 产品信息
  • 发表文章 (2)
  • 实验方案

PHKA1 Polyclonal antibody

PHKA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24279-1-AP

Print datasheet

Synonyms

PHKA, PHKA1



经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24279-1-AP is PHKA1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PHKA1 fusion protein Ag17998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase kinase, alpha 1 (muscle)
Synonyms PHKA, PHKA1
Calculated Molecular Weight 1223 aa, 137 kDa
Observed Molecular Weight 130 / 140 kDa
GenBank Accession NumberBC104944
Gene Symbol PHKA1
Gene ID (NCBI) 5255
RRIDAB_2879467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46020
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHKA1 antibody 24279-1-APDownload protocol
IHC protocol for PHKA1 antibody 24279-1-APDownload protocol
IF protocol for PHKA1 antibody 24279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanIHC

J Transl Med

Identification of molecular subtypes and a novel prognostic model of diffuse large B-cell lymphoma based on a metabolism-associated gene signature.

Authors - Jing He
mouseWB

Sci Adv

Lactate metabolism is essential in early-onset mitochondrial myopathy

Authors - Zhenkang Chen