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验证数据展示

  • WB analysis using 24658-1-AP

    WB analysis using 24658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24658-1-AP (PHKA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24658-1-AP

    IF Staining of HepG2 using 24658-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PHKA2 antibody (24658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human liver using 24658-1-AP

    IHC staining of human liver using 24658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PHKA2 antibody included
  • IHC staining of human liver using 24658-1-AP

    IHC staining of human liver using 24658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PHKA2 antibody included

PHKA2 Polyclonal antibody

PHKA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24658-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GSD9A, PHK, PHKA2, PHKLA, PYK, PYKL, XLG, XLG2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PHKA2 Ready-To-Use IHC Kit. PHKA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24658-1-AP targets PHKA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHKA2 fusion protein Ag9473 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase kinase, alpha 2 (liver)
别名 GSD9A, PHK, PHKA2, PHKLA, PYK, PYKL, XLG, XLG2
计算分子量138 kDa
观测分子量 138 kDa
GenBank蛋白编号BC014036
基因名称 PHKA2
Gene ID (NCBI) 5256
RRIDAB_2879659
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHKA2 antibody 24658-1-APDownload protocol
IHC protocol for PHKA2 antibody 24658-1-APDownload protocol
IF protocol for PHKA2 antibody 24658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Clin Invest

Imbalanced mitochondrial function provokes heterotaxy via aberrant ciliogenesis.

Authors - Martin D Burkhalter
View Article