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验证数据展示

  • IHC staining of human liver cancer using 15109-1-AP (same clone as 15109-1-PBS)

    IHC staining of human liver cancer using 15109-1-AP (same clone as 15109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    IF Staining of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    WB analysis of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    IP experiment of HEK-293 using 15109-1-AP (same clone as 15109-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300. This data was developed using the same antibody clone with 15109-1-PBS in a different storage buffer formulation.

PHKG2 Polyclonal antibody, PBS Only

PHKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15109-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15109-1-PBS targets PHKG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PHKG2 fusion protein Ag6829 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase kinase, gamma 2 (testis)
别名
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002541
基因名称 PHKG2
Gene ID (NCBI) 5261
RRIDAB_2165529
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15735
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.