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- KD/KO Validated
PHLDA1 Polyclonal antibody
PHLDA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse brain tissue, BXPC-3 cells, HeLa cells, MDA-MB-231 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18263-1-AP targets PHLDA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PHLDA1 fusion protein Ag13125 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pleckstrin homology-like domain, family A, member 1 |
| Synonyms | Proline- and glutamine-rich protein, PQ-rich protein, Pleckstrin homology-like domain family A member 1, PHRIP, DT1P1B11 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC018929 |
| Gene Symbol | PHLDA1 |
| Gene ID (NCBI) | 22822 |
| RRID | AB_2163284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WV24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IHC protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IF protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.
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Life Sci PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.
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Inflammation TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT-GSK-3β Pathway.
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Hum Exp Toxicol Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.
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Cell Death Dis The long non-coding RNA SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression.
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Mol Ther Nucleic Acids lncRNA HIF1A Antisense RNA 2 Modulates Trophoblast Cell Invasion and Proliferation through Upregulating PHLDA1 Expression. |
