验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse brain tissue, BXPC-3 cells, HeLa cells, MDA-MB-231 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18263-1-AP targets PHLDA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PHLDA1 fusion protein Ag13125 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology-like domain, family A, member 1 |
| 别名 | Proline- and glutamine-rich protein, PQ-rich protein, Pleckstrin homology-like domain family A member 1, PHRIP, DT1P1B11 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC018929 |
| 基因名称 | PHLDA1 |
| Gene ID (NCBI) | 22822 |
| RRID | AB_2163284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WV24 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IHC protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IF protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.
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Inflammation Multiple Machine Learning Identifies Key Gene PHLDA1 Suppressing NAFLD Progression | ||
Life Sci PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.
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Inflammation TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT-GSK-3β Pathway.
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Hum Exp Toxicol Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.
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Neuroreport Egr1 promotes Nlrc4-dependent neuronal pyroptosis through phlda1 in an in-vitro model of intracerebral hemorrhage
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