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PHPT1 Polyclonal antibody

PHPT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13112-1-AP

Print datasheet

Synonyms

Protein histidine phosphatase, PHP14, PHP, HSPC141, EC:3.9.1.3

验证数据展示

  • WB analysis of MCF-7 using 13112-1-AP

    WB analysis of MCF-7 using 13112-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13112-1-AP (PHPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13112-1-AP

    IF Staining of HeLa using 13112-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PHPT1 antibody (13112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, human kidney tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13112-1-AP targets PHPT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PHPT1 fusion protein Ag3778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphohistidine phosphatase 1
Synonyms Protein histidine phosphatase, PHP14, PHP, HSPC141, EC:3.9.1.3
Calculated Molecular Weight 125 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC024648
Gene Symbol PHPT1
Gene ID (NCBI) 29085
RRIDAB_2163737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHPT1 antibody 13112-1-APDownload protocol
IF protocol for PHPT1 antibody 13112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols