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  • Featured Product
  • KD/KO Validated

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12267-1-AP

Print datasheet

Synonyms

CRISP 9, CRISP9, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328

验证数据展示

  • WB analysis using 12267-1-AP

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12267-1-AP

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included
  • IHC staining of human liver cancer using 12267-1-AP

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PI16 antibody included

经过测试的应用

Positive WB detected inSKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12267-1-AP targets PI16 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PI16 fusion protein Ag2925 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidase inhibitor 16
Synonyms CRISP 9, CRISP9, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328
Calculated Molecular Weight 463 aa, 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC022399
Gene Symbol PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI16 antibody 12267-1-APDownload protocol
IHC protocol for PI16 antibody 12267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIHC,WB,CoIP

Cell Mol Biol Lett

Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Authors - Xiangqin Kuang
  • KD Validated
View Article