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PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30527-1-AP

Print datasheet

Synonyms

CRISP 9, CRISP9, DKFZp586B1817, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328

验证数据展示

  • WB analysis using 30527-1-AP

    WB analysis using 30527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 30527-1-AP

    IF Staining of SKOV-3 using 30527-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inCOLO 320 cells, rat heart tissue, SKOV-3 cells
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30527-1-AP targets PI16 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen PI16 fusion protein Ag32556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidase inhibitor 16
Synonyms CRISP 9, CRISP9, DKFZp586B1817, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328
Calculated Molecular Weight 463 aa, 49 kDa
Observed Molecular Weight 100 kDa, 49 kDa
GenBank Accession NumberBC022399
Gene Symbol PI16
Gene ID (NCBI) 221476
RRIDAB_3086350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI16 antibody 30527-1-APDownload protocol
IF protocol for PI16 antibody 30527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols