U2OS cells were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PI3 Kinase p110 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PI3 Kinase p110 Alpha antibody (67071-1-Ig, Clone: 1F6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PI3 Kinase p110 Alpha (67071-1-Ig, Clone:1F6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67071-1-PBS in a different storage buffer formulation.