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PI3 Kinase p85 Beta Monoclonal antibody, PBS Only

PI3 Kinase p85 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3B6

Cat no : 67644-1-PBS

Print datasheet

Synonyms

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

验证数据展示

  • WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3R2 antibody included
  • IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3R2 antibody included
  • IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

    IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.

产品信息

67644-1-PBS targets PI3 Kinase p85 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PI3 Kinase p85 Beta fusion protein Ag14369 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Synonyms p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2
Calculated Molecular Weight 728 aa, 82 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014170
Gene Symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00459
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).