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Species Reactivity
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验证数据展示

  • WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3R2 antibody included
  • IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIK3R2 antibody included
  • IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

    IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

PI3 Kinase p85 Beta Monoclonal antibody, PBS Only

PI3 Kinase p85 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67644-1-PBS

产品说明书

CloneNo. 2B3B6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PIK3R2 Ready-To-Use IHC Kit. PIK3R2 primary antibody included.

产品信息

67644-1-PBS targets PI3 Kinase p85 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PI3 Kinase p85 Beta fusion protein Ag14369 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 2 (beta)
别名 p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2
计算分子量 728 aa, 82 kDa
观测分子量 85 kDa
GenBank蛋白编号BC014170
基因名称 PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
RRIDAB_2882844
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00459
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).