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验证数据展示

  • WB analysis using 21708-1-AP

    WB analysis using 21708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21708-1-AP (PI3 Kinase p110 Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 21708-1-AP

    IF Staining of LNCaP using 21708-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using PI3 Kinase p110 Delta antibody (21708-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

PI3 Kinase p110 Delta Polyclonal antibody

PI3 Kinase p110 Delta Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21708-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

PI3K p110(delta), PIK3CD, PI3K delta, PI3 kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, RAW 264.7 cells
Positive IF/ICC detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21708-1-AP targets PI3 Kinase p110 Delta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse, rat
免疫原 PI3 Kinase p110 Delta fusion protein Ag16326 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, delta polypeptide
别名 PI3K p110(delta), PIK3CD, PI3K delta, PI3 kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
计算分子量 1044 aa, 120 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC132919
基因名称 PIK3CD
Gene ID (NCBI) 5293
RRIDAB_3085666
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Delta antibody 21708-1-APDownload protocol
IF protocol for PI3 Kinase p110 Delta antibody 21708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Mol Life Sci

FAM76B regulates PI3K/Akt/NF-κB-mediated M1 macrophage polarization by influencing the stability of PIK3CD mRNA

Authors - Juan Wang
View Article
ratIF

J Ethnopharmacol

Uncovering the mechanisms of Zhubi decoction against rheumatoid arthritis through an integrated study of network pharmacology, metabolomics, and intestinal flora

Authors - Jing Liu
View Article